BOTA 32033

>< Type/ Status : Core
>< Course title : Molecular Genetics and Gene Technology
>< Prerequisite : BIOL 11032
>< Objective : By the end of the course the student will demonstrate knowledge and understanding of the nature and expression of genes, essential techniques used for gene manipulation and application of biotechnological approaches for plant improvement.
>< Course content:

Structure and organization of prokaryotic and eukaryotic genomes. Structure and function of prokaryotic genes. Regulation of prokaryotic gene expression. Plasmids and gene transfer among bacteria. Introduction to eukaryotic genes and their transcription. Isolation and modification of genes. Construction and screening of genomic and DNA libraries. Gene cloning and transgenic organisms. Transposable genetic elements and their use in gene modifications. Production of transgenic plants using genetic engineering and use of Ti plasmid in plant genetic engineering.

>< Methodology: Lectures, practicals and tutorials.
>< Scheme of evaluation: End of course unit written examination.
>< Recommended reading:
01. Lewin, B (2000). Genes VII. Oxford University.
02. Griffiths, A J F, Miller, J H, Suzuki, D T and Gelbart, W M (1996). An Introduction to Genetic Analysis. 6th Edition. W H Freeman.
03. Sambrook, J, Fritsch, E F and Maniatis, T (1989). Molecular Cloning. A Laboratory Manual. Cold Spring Harbour Laboratory.
04. Jones, P G and Sutton, J M (1997). Plant Molecular Biology. John Wiley & Sons.
05. Wolfe, S L (1995). Introduction to Cell and Molecular Biology. Wordsworth.

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