MIBI 21014

>< Type/Status : Core
>< Title : Microbial Genetics and Microbial Physiology & Biochemistry
>< Pre-requisite : MIBI 12014 & MIBI 12022
>< Co-requisite : MIBI 21022
>< Objectives : At the end of the course unit students will be able to demonstrate the knowledge and understanding of
• the fundamental and advance concepts of microbial genetics,
• the principles of the recombinant DNA technology and the bacterial transformation,
• the application of recombinant DNA organisms and their usage in Agricultural, Medical & Industrial activities,
• the different methods of carbohydrate metabolism present in bacteria,
• the production of various metabolic products via fermentation.
>< Course content:

Microbial Genetics

Structure and function of prokaryotic genetic material: Chromosome structure, Prokaryotic gene and gene code.

Molecular basis of inheritance: Regulation of bacterial gene expression: Induction, repression and Catabolic repression. Mutations: Types, detection and selection of mutations, Mutagens, Expression of mutations. Genetic transfer and recombination: Plasmids, Transposable elements, Transformation, Conjugation and Transduction.

Genetic manipulations: recombinant DNA technology, DNA cloning and cloning vectors, Expression of foreign genes in bacteria. Genetic engineering: Industrial, Agricultural and Medical applications.

Microbial Physiology & Biochemistry

Nutrition of bacteria: Major & minor bio – elements, nutrients as energy source, growth factor requirements of bacteria.

Carbohydrate metabolism in bacteria: EMP, HMP, ED & phosphoketolase pathways,

Hetorotrophic generation of ATP in bacteria: fermentation, Autotrophic generation of ATP: Chemolithotrophy, bacterial photosynthesis. Transport of nutrients in microorganisms.

Biosynthesis of macromolecules in microorganisms: Amphibolic pathways, biosynthesis of proteins and lipids, Calvin cycle. Biodegradation of macromolecules.

>< Methodology : A combination of lectures and tutorials.
>< Scheme of Evaluation : 100% end of course examination.
>< Recommended Reading:
1. Freifelder, D (1998). Molecular Biology. Jones & Bartlett Publishers, Inc.
2. Singer, M. and Berg, P. (1991). Genes and Genome. University Science Books, U.S.A.
3. Turner, P.C ; McLennan, A.G; Bates, A.D and White, M.R.S (1997). Instant Notes in Molecular Biology. Springer-Verlag New York.
4. Gottschalk, G (1986). Bacterial Metabolism. Springer-Verlag, New York.
5. Mandelstam, J.;.McQuillen, K. and. Dawes, I. (1983). Biochemistry of Bacterial Growth. Blackwell Scientific Publications.
6. Mohan, S.; Dow, C. and Cole, J.A. (1992). Prokaryotic Structure and function: a new perspective. Cambridge University Press

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