MIBI 21022

>< Type/Status : Core
>< Title : Microbial genetics and Microbial physiology and Biochemistry Laboratory
>< Pre-requisite : MIBI 12014 & MIBI 12022
>< Co-requisite : MIBI 21014
>< Objectives : At the end of the course unit students will be able to demonstrate the knowledge and understanding of
• the detection & differentiation of microorganisms using DNA based techniques,
• the biologically important components in the laboratory,
• some physiological activities of microorganisms,
• the potential usage of some microbial metabolic activities/processes in industries.
>< Course content :

Laboratory exercises to illustrate the contents and special features emphasized in lecture course MIBI 21014. Preparation of bacterial Genomic DNA, Preparation of bacterial plasmid DNA, Digestion of DNA and analysis of the digestions by Gel electrophoresis: Restricted Fragment Length Polymorphism (RFLP) analysis, Bacterial transformation and cloning of DNA, Polymerase Chain Reaction (PCR) for the detection of important Bacterial species and some viruses. Enzyme linked Immunosorbent Assay (ELISA) test for the identification of the viruses

Determination of bacterial enzymes, chromatography techniques

>< Methodology : Laboratory practical and demonstration classes.
>< Scheme of Evaluation : End of course examination.
>< Recommended Reading:
1. Anderson, P and Ganetzky, B. (1997). An electronic companion to genetics workbook. Cogito Learning Media, Inc., New York
2. Creighton, T.E. (1990). Protein Structure a Practical Approach. IRL Press, New York,
3. John G. Holt (1994) Bergy's Manual of Determinative Bacteriology. Williams & Wilkins, Baltimore
4. Sambrook, J., Frtsch, E.F and Maniatis, T. (1989). Molecular Cloning- A Laboratory Manual. Vol. 1, 2, and 3. Cold Spring Harbor.

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