<BOTA 21013> <BOTA 21022> <BOTA 22034> <BOTA 22042>

BOTA 21022

>< Type/ Status : Core
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Course title : Plant Physiology Laboratory
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Corequisite : BOTA 21013
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Objective: By the end of the course, the student will be able to demonstrate essential knowledge, understanding and skills needed in studying plant functions.
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Course content:

Work with aqueous solutions and buffers. Determination of water potential and solute potential. Measurement of transpiration. Stomatal movement. Separation of photosynthetic pigments. Quantitative analysis of chlorophyll. Hill reaction. Acid accumulation of CAM plants. Oxygen liberation in photosynthesis. Permeability of cell membranes to different ions and molecules. Mineral deficiency symptoms in plants. Shoot and root growth. Leaf area measurements. Hormonal action. Seed viability and germination tests.
Studies on membrane permeability. Studies on enzyme activity and kinetics.

>< Methodology: Five hours of laboratory exercises per week.
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Scheme of evaluation: End of course practical examination.
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Recommended reading:
01. Ross, L W (1974). Plant Physiology Laboratory Manual. Wadsworth.
02. Frais, F (1972). Practical Biochemistry - An Introductory Course. Butterworths.
03. Plummer, D T ( 1971). An Introduction to Practical Biochemistry. McGraw-Hill

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