CHEM 21033

>< Type/Status : Compulsory
>< Title : Analytical Chemistry
>< Pre-requisites : CHEM 12032, CHEM 12042
>< Objectives : To provide the fundamentals of analytical methods in chemistry.
>< Course Content :

Analytical Spectroscopy

Interaction of electromagnetic radiation with matter. emission, absorption and fluorescence Processes of radiation. atomic absorption, emission and fluorescence spectrometry. molecular absorption and luminescence spectrometry.

Electro-analytical Techniques

Potentiometry and ion selective electrodes. Electrogravimetry and Coulometry. Polorography and modified voltammetric techniques.

Analytical Separation Techniques

Solvent extraction . Thin layer chromatography. Gas chromatography,Liquid chromatography, and Electrophoresis

Laboratory Experiments in Analytical Chemistry

Experiments based on analytical spectrometry, electro-analytical techniques and chromatographic techniques

Organic Spectroscopy

Electromagnetic radiation. Ultraviolet–visible spectroscopy (Beer Lambert’s Law, chromophores and effect of conjugation and solvents), Woodward rules. Infrared spectroscopy (functional group frequencies), Nuclear magnetic resonance spectroscopy, 1H NMR spectroscopy (chemical shifts, first order spin-spin coupling, deuterium exchange, long range coupling), 13C NMR (chemical shifts, off resonance and proton decoupled 13C NMR), Mass spectroscopy (molecular ion peak, base peak, isotopic peaks, metastable peaks, fragmentation and rearrangements). Structure elucidation of organic compounds by spectral analysis.

Practicals

Effect of pH on UV absorption of amines and phenols, determination of impurities using UV spectroscopy. IR spectra of organic compounds. Structure elucidation of organic compounds by spectral analysis.

>< Process : A combination of lectures, tutorials and laboratory experiments.
>< Scheme of Evaluation : End of course unit examination on theory and continuous assessment on laboratory experiments.
>< Recommended Reading :
1. Skoog, D. A., West, D. M. and Holler, F. J. (1996), Fundamentals of Analytical Chemistry, 7th edition, Saunders.
2. Christian, G. D. (1994), Analytical Chemistry, 5th edition, John Wiley.
3. Skoog, D. A., West, D. M. and Holler, F. J. (1994), Analytical Chemistry, An Introduction, Saunders.
4. Skoog, D. A., Holler, F. J. and Nieman, T. M. (1998), Principles of Instrumental Analysis, 5th edition, Saunders.
5. Williams, D. H. (1989), Spectroscopic Methods in Organic Chemistry, 4th edition, McGraw Hill.
6. Pavia, D. L., Lapman, G. M. and Kriz, G. S. (1979), Introduction to Spectroscopy, Saunders.
7. Silverstein, R. M., Bassler, G. S. and Morril, T. C. (1991), Spectrometric Identification of Organic Compounds, 5th edition, John Wiley.

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