MBBT 41016

>< Type/Status : Compulsory
>< Course Title : Advanced Microbial Genetics
>< Objectives: At the end of the course, the student will be able to demonstrate
(i) comprehensive knowledge and understanding of prokaryotic gene expression and gene transfer, and
(ii) ability to interpret experimental strategies involving gene transfer mechanisms
>< Course content:

Prokaryotic gene expression: features of bacterial promoters, molecular details of transcription initiations and termination. Regulation of gene expression: transcriptional regulation in bacteria; repressors and activators, positive and negative regulation, attenuation and feed back inhibition. Mechanism of DNA uptake during transformation. Genetics of bacteriophages: lytic development cycle, phage DNA replication. Generalized transduction, lysogeny and specialized transduction. Transposition and nonhomologous recombination, DNA repair and mutagenesis.

>< Methodology: Lectures, tutorials and practical assignments.
>< Scheme of evaluation: End of course written examination and practical assignments.
>< Recommended reading:
01. Synder, L. and Champness, W. (1997). Molecular Genetics of Bacteria. American Society for Microbiology.
02. Lewin, B. (2000). Genes VII. Oxford University.
03. Freifelder, D. (1997). Microbial Genetics. Jones and Bartlet Publishers.

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