MBBT 41044

>< Type/Status : Compulsory
>< Course title : Genetic Manipulation of Microorganisms
>< Objectives: Having completed the course, the student will be able to
(i) understand the potential of genetic manipulation of microorganisms in biotechnology, and
(ii) demonstrate understanding of appropriate applications of these manipulations.
>< Course content:

Exclusive expression of foreign genes in bacteria. Deletion mapping of protein functional domains. Regulation of plasmid replication. Transposon mutagenesis and in vivo cloning. Suicide vectors and their use in bacterial genetic manipulations. Gene replacement and reverse genetics. Genetic modification of bacteriophages in the development of vectors. Use of viral genetic elements in recombinant DNA techniques. Gene cloning, manipulation and targeting in yeast.

>< Methodology: Lectures, tutorials and practical assignments.
>< Scheme of evaluation: End of course written examination and practical assignments.
>< Recommended reading:
01. Synder, L. and Champness, W. (1997). Molecular Genetics of Bacteria. American Society for Microbiology.
02. Freifelder, D. (1997). Microbial Genetics. Jones and Bartlet.

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